Information On Protein Purification Services

By | June 12, 2021

Protein purification services are available to anyone who needs specific antibodies or recombinant proteins for research and development. If your lab isn't equipped to make recombinant proteins at home, this can be an attractive option. 

This service requires you to provide a preferred consistency and expression system; This, in turn, delivers quality-tested protein with accompanying documentation in just a few weeks. You can get the details on protein expression help by browsing the internet.

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Recombinant antibodies and proteins are essential for the development of immunoassays, high-performance screens, multiplex assays, structural analysis and detection of active substances. Natural protein isolates cannot give high enough yields for this application. Therefore, the ability to order them on demand is very useful for the advancement of research and development.

Protein purification service steps

Determine the order of interest

  • Choose an optimized cloning vector and expression system

  • Specify open reading frames, untranslated regions and clone sites

  • A vector is a plasmid or virus designed for gene expression in cells. It is used to introduce the desired gene into target cells and contains regulatory sequences that act as enhancer and promoter regions. The vector must match the expression system. 

Optimize genetic code

  • Commercial optimization software available

  • The genetic code has degenerated; each amino acid has several sequences that code for it. This is known as codon bias; there are 64 codons (61 + 3 stop codons) but only 20 amino acids are translated.

  • Expression of proteins in heterologous hosts (organisms that do not naturally express proteins) may require gene optimization to alter the gene sequence to contain the host organism's preferred codon.

Other parameters that affect protein expression:

  • Transcriptional level: GC content, consensus junction site, crypto junction site, SD sequence, TATA box, terminal signal, the artificial recombination site

  • MRNA levels: RNA instability motifs, ribosomal entry points, repetitive sequences

  • Translation level: codon use, premature poly(A), ribosomal entry point, secondary structure